7VCP
Frischella perrara beta-fructofuranosidase in complex with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 66.255, 66.255, 463.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.800 - 2.000 |
R-factor | 0.18083 |
Rwork | 0.179 |
R-free | 0.21228 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vco |
RMSD bond length | 0.008 |
RMSD bond angle | 1.454 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.094 | 0.714 |
Rpim | 0.022 | 0.166 |
Number of reflections | 42744 | 6054 |
<I/σ(I)> | 21.8 | 4.8 |
Completeness [%] | 100.0 | |
Redundancy | 18.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2-propanol, PEG 600, PEG 3350, ammonium acetate, HEPES-NaOH |