7VCL
structure of viral protein BKRF4 in complex with H2A-H2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.474, 78.040, 105.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.853 - 3.200 |
| R-factor | 0.2339 |
| Rwork | 0.229 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k01 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.558 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
| Rmerge | 0.171 | 0.059 | 0.747 |
| Rmeas | 0.179 | 0.062 | 0.790 |
| Rpim | 0.051 | 0.018 | 0.249 |
| Total number of observations | 54863 | ||
| Number of reflections | 4730 | 276 | 208 |
| <I/σ(I)> | 3.2 | ||
| Completeness [%] | 99.1 | 99.6 | 92 |
| Redundancy | 11.6 | 10.7 | 9.2 |
| CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium chloride; 0.1 M HEPES pH 7.5; 35% MPD |
