7VC7

The structure of beta-xylosidase from Phanerochaete chrysosporium(PcBxl3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-12-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	79.367, 91.071, 106.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.079 - 3.080
R-factor	0.2068
Rwork	0.204
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vc6
RMSD bond length	0.003
RMSD bond angle	0.704
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.18.2-3874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.080	3.190
High resolution limit [Å]	3.080	3.080
Number of reflections	14756	1450
<I/σ(I)>	7.82
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.985	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	525mM Malic acid (pH7.0), 20% v/v PEG 3350