7VC6

The structure of beta-xylosidase from Phanerochaete chrysosporium(PcBxl3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-12-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	79.667, 91.292, 107.082
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.438 - 2.540
R-factor	0.1784
Rwork	0.175
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ae6
RMSD bond length	0.003
RMSD bond angle	0.727
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.18.2-3874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.440	2.631
High resolution limit [Å]	2.540	2.540
Number of reflections	26406	2587
<I/σ(I)>	11.52
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.995	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.2M Lithium sulfate, 0.1M Tris (pH8.5), 40% v/v PEG 400