7VBS

Structure of the AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 65
Unit cell lengths	108.678, 108.678, 40.375
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.105 - 1.998
R-factor	0.2004
Rwork	0.198
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5exs
RMSD bond length	0.003
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.105	2.070
High resolution limit [Å]	1.998	1.998
Number of reflections	18704	1818
<I/σ(I)>	18.33
Completeness [%]	99.7
Redundancy	11.3
CC(1/2)	1.000	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	11% (w/v) Polyethylene glycol 6000, 100 mM Tris-HCl pH 8.1, 100 mM Ammonium phosphate dibasic