7VBF
1.3 Angstrom Resolution Crystal Structure of SARS-CoV-2 Nucleocapsid dimerization domain, pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.710, 65.130, 84.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.147 - 1.300 |
| R-factor | 0.185605509437 |
| Rwork | 0.185 |
| R-free | 0.20391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wji |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.839 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.180 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.549 | |
| Number of reflections | 112547 | 59926 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.1 | |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4000 |
