7VA6

PaOrn Oligoribonuclease D11A mutant with RNA GU complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 41
Unit cell lengths	82.993, 82.993, 192.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.750 - 2.100
R-factor	0.168
Rwork	0.167
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v9z
RMSD bond length	0.007
RMSD bond angle	0.751
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.056	0.296
Number of reflections	38215	1918
<I/σ(I)>	22.74	2.44
Completeness [%]	100.0	99.9
Redundancy	6.4	5.5
CC(1/2)	0.996	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	1.2M (NH4)2HPO4, 0.1M imidazole pH 8.0