7VA3

PaOrn Oligoribonuclease D11A mutant with substrate pGpG complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 41
Unit cell lengths	83.223, 83.223, 192.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.610 - 1.800
R-factor	0.1728
Rwork	0.172
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v9z
RMSD bond length	0.006
RMSD bond angle	0.697
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.072	0.343
Number of reflections	60258	2938
<I/σ(I)>	28.44	1.76
Completeness [%]	99.9	98.1
Redundancy	6.1	4.4
CC(1/2)	0.992	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	1.2M (NH4)2HPO4, 0.1M imidazole pH 8.0