7VA2

PaOrn Oligoribonuclease D11A mutant with product GMP complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 41
Unit cell lengths	82.627, 82.627, 192.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.910 - 2.300
R-factor	0.1965
Rwork	0.194
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v9z
RMSD bond length	0.007
RMSD bond angle	0.889
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.092
Number of reflections	30350	1541
<I/σ(I)>	15.61	1.15
Completeness [%]	99.8	100
Redundancy	4.8	4.4
CC(1/2)	0.969	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	1.2M (NH4)2HPO4, 0.1M imidazole pH 8.0