7V9P
Crystal structure of the lanthipeptide zinc-metallopeptidase EryP from saccharopolyspora erythraea in intermediate state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.420, 66.630, 153.740 |
| Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
| Resolution | 31.060 - 1.800 |
| R-factor | 0.1903 |
| Rwork | 0.190 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v9n |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.740 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.510 |
| Rmeas | 0.057 | 0.601 |
| Rpim | 0.022 | 0.308 |
| Number of reflections | 328585 | 11286 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 98.9 | 90.4 |
| Redundancy | 6.1 | 3.6 |
| CC(1/2) | 0.999 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.2 M Sodium chloride, 0.1 M Tris ,pH 8.0, 20% w/v PEG 4000 |
