7V9N

Crystal structure of the lanthipeptide zinc-metallopeptidase EryP from saccharopolyspora erythraea in closed state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	153.577, 153.577, 98.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.080 - 1.900
R-factor	0.1544
Rwork	0.154
R-free	0.18070
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.126	0.758
Rmeas	0.128	0.772
Rpim	0.025	0.149
Number of reflections	92705	4506
<I/σ(I)>	25.2
Completeness [%]	100.0	99.8
Redundancy	19.4
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	0.1 M calcium acetate, 0.1 M sodium acetate, pH 4.5, 10% w/v PEG 4000