7V9F
Selenomethionine mutant (L740Sem) of BEN4 domain of protein Bend3 with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9788 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 96.831, 96.831, 141.693 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.153 - 2.500 |
R-factor | 0.1797 |
Rwork | 0.177 |
R-free | 0.22560 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.814 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.082 | |
Number of reflections | 25183 | 13812 |
<I/σ(I)> | 38.9 | |
Completeness [%] | 99.8 | |
Redundancy | 21 | |
CC(1/2) | 0.082 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM sodium citrate/Citric acid pH 4.0, 200 mM Sodium citrate tribasic, and 17% PEG 3350 |