7V8T

Crystal structure of class II pyruvate aldolase from Pseudomonas aeruginosa.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	72.952, 131.077, 84.202
Unit cell angles	90.00, 106.80, 90.00

Refinement procedure

Resolution	46.540 - 2.480
R-factor	0.1876
Rwork	0.186
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vwt
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.480	2.500
Rmeas	0.241
Rpim	0.094	0.566
Number of reflections	51389	2440
<I/σ(I)>	6.4
Completeness [%]	96.6
Redundancy	6
CC(1/2)		0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	200mM sodium iodide, 24% (w/v) PEG 3350