7V8P
Crystal Structure of the MukE dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-08-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.560, 64.360, 62.250 |
Unit cell angles | 90.00, 116.65, 90.00 |
Refinement procedure
Resolution | 32.656 - 2.440 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.26280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3euh |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.640 | 55.640 | 2.540 |
High resolution limit [Å] | 2.439 | 8.790 | 2.440 |
Rmerge | 0.094 | 0.051 | 0.924 |
Rmeas | 0.101 | 0.055 | 0.995 |
Rpim | 0.037 | 0.021 | 0.366 |
Total number of observations | 1242 | 6932 | |
Number of reflections | 8635 | 199 | 958 |
<I/σ(I)> | 12 | 29.6 | 2.2 |
Completeness [%] | 99.3 | 98.9 | 98.6 |
Redundancy | 7.4 | 6.2 | 7.2 |
CC(1/2) | 0.998 | 0.996 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2 M Sodium chloride;0.1 M IBS pH 8.5;25% w/v Polyethylene glycol 3,350 |