7V8K
Toxoplasma gondii Prolyl-tRNA Synthetase (TgPRS) in Complex with L-Proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-02 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9197 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.196, 81.673, 104.660 |
Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
Resolution | 68.291 - 2.470 |
R-factor | 0.1693 |
Rwork | 0.167 |
R-free | 0.21930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xif |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.000 | 2.510 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmeas | 0.204 | 1.429 |
Number of reflections | 45661 | 2263 |
<I/σ(I)> | 8.2 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 6.6 |
CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Amino acids (0.2M DL-Glutamic acid monohydrate, 0.2M DL-Alanine, 0.2M Glycine, 0.2M DL-Lysine monohydrochloride, 0.2M DL-Serine), 0.1 M Buffer (Imidazole, MES monohydrate), 30 % v/v Precipitant (40% v/v Ethylene glycol, 20% w/v PEG 8000) |