7V8E

Crystal structure of IpaH1.4 LRR domain bound to HOIL-1L UBL domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	31.809, 142.684, 68.598
Unit cell angles	90.00, 94.03, 90.00

Refinement procedure

Resolution	23.780 - 1.900
R-factor	0.2032
Rwork	0.201
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dbg
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS (20190606)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	1.940
High resolution limit [Å]	1.900	1.900
Rpim	0.025	0.283
Number of reflections	47800	3094
<I/σ(I)>	16.3	2.3
Completeness [%]	99.8	99.5
Redundancy	6.3
CC(1/2)	0.998	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.2 M (NH4)2SO4, 0.1 M Tris-HCl (pH 8.5), and 25% (w/v) PEG3350