7V8E
Crystal structure of IpaH1.4 LRR domain bound to HOIL-1L UBL domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.809, 142.684, 68.598 |
Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
Resolution | 23.780 - 1.900 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dbg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | XDS (20190606) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.380 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rpim | 0.025 | 0.283 |
Number of reflections | 47800 | 3094 |
<I/σ(I)> | 16.3 | 2.3 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.3 | |
CC(1/2) | 0.998 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 289 | 0.2 M (NH4)2SO4, 0.1 M Tris-HCl (pH 8.5), and 25% (w/v) PEG3350 |