7V86

Cryo-EM structure of SARS-CoV-2 S-Kappa variant (B.1.617.1) in complex with Angiotensin-converting enzyme 2 (ACE2) ectodomain, three ACE2-bound form

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.8
Software	cryoSPARC,EPU
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION