7V7K
Crystal structure of Antibody 16A in complex with MUC1 Glycopeptide(GlycoST)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-09-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.052, 136.622, 80.993 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.700 - 2.200 |
R-factor | 0.2034 |
Rwork | 0.201 |
R-free | 0.25070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhy |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.129 | 0.730 |
Rpim | 0.072 | 0.490 |
Number of reflections | 21331 | 1731 |
<I/σ(I)> | 10.5 | |
Completeness [%] | 93.4 | |
Redundancy | 1.6 | |
CC(1/2) | 0.994 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 1.0 M Lithium chloride, 0.1 M Tris pH 8.5, 20%(w/v) PEG 6000 |