7V6W
Crystal structure of heterohexameric Sa2YoeB-Sa2YefM complex bound to 26bp-DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 236.609, 66.358, 129.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.950 - 2.550 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.23090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v5y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 66262 | 3168 |
<I/σ(I)> | 17.4 | |
Completeness [%] | 98.6 | |
Redundancy | 6.6 | |
CC(1/2) | 0.993 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.02 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate pH 7.0, 15% v/v 2-Propanol, 0.001 M Hexamine cobalt (III) chloride, 0.001 M Spermine |