7V6M
Crystal structure of lacto-N-biosidase TnX from Tynzenella nexilis, lacto-N-biose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 55.205, 71.782, 161.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.610 - 1.920 |
| R-factor | 0.17165 |
| Rwork | 0.170 |
| R-free | 0.20949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gqf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.610 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.110 | 0.646 |
| Rmeas | 0.120 | 0.726 |
| Rpim | 0.047 | 0.325 |
| Number of reflections | 50268 | 3314 |
| <I/σ(I)> | 11.3 | 2.3 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.4 | 4.7 |
| CC(1/2) | 0.997 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium formate, 20% (w/v) PEG 3350 |
