7V6I

Crystal structure of lacto-N-biosidase BsaX from Bifidobacterium saguini, lacto-N-biose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	113.240, 113.240, 79.097
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.080 - 2.510
R-factor	0.15995
Rwork	0.157
R-free	0.22759
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gqf
RMSD bond length	0.009
RMSD bond angle	1.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.610
High resolution limit [Å]	2.510	2.510
Rmerge	0.184	0.695
Rmeas	0.194	0.732
Rpim	0.061	0.228
Number of reflections	20374	2262
<I/σ(I)>	11.2	3.8
Completeness [%]	100.0	100
Redundancy	10	10.2
CC(1/2)	0.995	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.5M ammonium sulfate, 0.1M sodium acetate (pH 4.6), 30% (w/v) PEG 4000