7V6E
DREP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2018-03-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.460, 125.355, 168.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.110 - 3.000 |
| R-factor | 0.2092 |
| Rwork | 0.205 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d2k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.110 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 0.053 |
| Number of reflections | 45952 | 2406 |
| <I/σ(I)> | 3.74 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M citric acid pH 4.0, 0.8M ammonium sulfate |
