7V67

Crystal Structure of Enolase1 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	265.204, 62.183, 111.815
Unit cell angles	90.00, 109.92, 90.00

Refinement procedure

Resolution	33.114 - 2.000
R-factor	0.1789
Rwork	0.177
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2al2
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.114	50.000	2.071
High resolution limit [Å]	2.000	5.170	2.000
Rmerge	0.034	0.084	0.233
Rmeas	0.049	0.094	0.233
Rpim	0.034	0.041	0.330
Number of reflections	116013	6965	11527
<I/σ(I)>	12.79
Completeness [%]	98.9	99.7	99.86
Redundancy	5.5	5.2	5.6
CC(1/2)		0.990	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350