7V64
Crystal structure of Antibody 16A in complex with MUC1 Glycopeptide(GlycoT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.534, 81.225, 137.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.610 - 1.560 |
R-factor | 0.1803 |
Rwork | 0.179 |
R-free | 0.20280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhy |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.610 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmeas | 0.855 | |
Rpim | 0.258 | |
Number of reflections | 62756 | 5598 |
<I/σ(I)> | 23.7 | 2.2 |
Completeness [%] | 98.1 | |
Redundancy | 6.4 | |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 1.0 M Lithium chloride, 0.1 M Tris pH 8.5, 20% PEG 6000 |