7V5Z
Crystal structure of heterotetrameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.694, 92.243, 108.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.760 - 1.990 |
R-factor | 0.1747 |
Rwork | 0.172 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v5y |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 23229 | 1152 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 12.5 | 10.4 |
CC(1/2) | 0.991 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.2 M Succinic acid pH 7.0, 20% w/v Polyethylene glycol 3350 |