7V5Y
Crystal structure of hexameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.989, 66.984, 82.361 |
Unit cell angles | 90.00, 105.21, 90.00 |
Refinement procedure
Resolution | 48.560 - 2.250 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a6q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.558 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 26095 | 1296 |
<I/σ(I)> | 26.7 | |
Completeness [%] | 99.9 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.1 M Ammonium tartrate dibasic pH 7.0, 12% w/v Polyethylene glycol 3350 |