7V5V

LysR family transcriptional regulator RipR from Salmonella Typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2021-07-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0448
Spacegroup name	P 1 21 1
Unit cell lengths	66.405, 72.415, 91.612
Unit cell angles	90.00, 104.62, 90.00

Refinement procedure

Resolution	39.260 - 2.370
R-factor	0.2385
Rwork	0.236
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
Data reduction software	HKL-2000 (v722)
Data scaling software	HKL-2000 (v722)
Phasing software	MOLREP (7.1.012)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.100	0.055	0.362
Rmeas	0.109	0.059	0.396
Rpim	0.043	0.022	0.159
Number of reflections	42095	2206	2062
<I/σ(I)>	17.6
Completeness [%]	98.7	99.4	98.2
Redundancy	6.1	7.1	5.9
CC(1/2)		0.997	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.2 M Sodium formate pH 5.5, 19% (w/v) PEG 3,350