7V5P
The dimeric structure of G80A/H81A myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.527, 62.735, 83.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.710 - 1.160 |
R-factor | 0.19697 |
Rwork | 0.197 |
R-free | 0.20562 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vm9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.369 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.350 | 1.180 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmerge | 0.034 | |
Number of reflections | 101566 | 4675 |
<I/σ(I)> | 23.1 | |
Completeness [%] | 97.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M Sodium acetate, 0.1 M Tris-HCl, 10% (w/v) PEG 6,000 |