7V5H
VcOrn native structure with N terminal tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.522, 84.522, 55.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.670 - 1.700 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n6a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.041 | 0.395 |
| Number of reflections | 25735 | 1259 |
| <I/σ(I)> | 25.9 | 2.76 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 289 | 2.0M sodium formate, 0.1M sodium acetate trihydrate pH 4.6 |
