7V4Z
Structure of Horcolin native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 69.179, 94.419, 238.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.866 - 1.160 |
| Rwork | 0.181 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.974 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.830 | 1.230 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.092 | 0.420 |
| Number of reflections | 513816 | 69425 |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 96.8 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.990 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000 |
