7V4W
Crystal structure of Antibody 16A in complex with MUC1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-09-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.557, 63.370, 88.681 |
| Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
| Resolution | 39.250 - 2.100 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yhy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.841 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.250 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.129 | 0.390 |
| Rpim | 0.068 | 0.241 |
| Number of reflections | 23401 | 1379 |
| <I/σ(I)> | 25.3 | 3.4 |
| Completeness [%] | 91.5 | |
| Redundancy | 1.8 | 1.3 |
| CC(1/2) | 0.964 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Di-sodium hydrogen phosphate 0.2M, PEG 3350 20% |
