7V4O

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	80
Detector technology	CCD
Collection date	2020-07-17
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	150.839, 39.035, 76.591
Unit cell angles	90.00, 119.34, 90.00

Refinement procedure

Resolution	25.030 - 1.650
R-factor	0.1817
Rwork	0.180
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p7x
RMSD bond length	0.006
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.030	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	46721	4689
<I/σ(I)>	32.3
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Lithium sulfate monohydrate 0.1M ADA pH 6.5 12% PEG 4K 2% v/v 2-Propanol