7V4N

Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-03-27
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 43 21 2
Unit cell lengths	82.243, 82.243, 104.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 2.200
R-factor	0.1932
Rwork	0.187
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p7x
RMSD bond length	0.015
RMSD bond angle	1.757
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.830	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	18830	18812
<I/σ(I)>	24.9
Completeness [%]	100.0
Redundancy	14.4
CC(1/2)	0.874	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.2M Sodium chloride 0.1 M Tris pH 8.5