7V4M
Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2021-04-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.184, 77.568, 68.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.900 |
R-factor | 0.1911 |
Rwork | 0.186 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v4f |
RMSD bond length | 0.007 |
RMSD bond angle | 0.919 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 16089 | 1596 |
<I/σ(I)> | 23.6 | |
Completeness [%] | 100.0 | |
Redundancy | 5.3 | |
CC(1/2) | 0.780 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Calcium acetate 0.1M MES pH 6.4 9% V/V PEG 4K 10 mg |