7V3X
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.492, 205.803, 186.177 |
| Unit cell angles | 90.00, 115.14, 90.00 |
Refinement procedure
| Resolution | 49.220 - 3.100 |
| R-factor | 0.2777 |
| Rwork | 0.275 |
| R-free | 0.33970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.102 | 0.744 |
| Number of reflections | 228464 | 11426 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 313 | Acetatic acid, Sodium formate |
