7V3N
The complex structure of soBcmB-D307A and its natural precursor 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97913 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 114.460, 114.460, 55.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.935 - 1.850 |
R-factor | 0.167576180049 |
Rwork | 0.166 |
R-free | 0.19361 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v3o |
RMSD bond length | 0.006 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.940 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.090 | 0.561 |
Number of reflections | 32011 | 2240 |
<I/σ(I)> | 22.1 | |
Completeness [%] | 99.7 | |
Redundancy | 18.8 | |
CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES monohydrate pH 6.5, 1.6M Ammonium sulfate, 10% 1.4-dioxane |