7V3K
crystal structure of MAJ1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 114.864, 114.864, 264.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.890 - 2.490 |
| R-factor | 0.1897 |
| Rwork | 0.187 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h6g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.330 | 0.827 |
| Rpim | 0.109 | 0.311 |
| Number of reflections | 60324 | 2958 |
| <I/σ(I)> | 10 | 1.98 |
| Completeness [%] | 96.1 | 96.7 |
| Redundancy | 9.6 | 9.4 |
| CC(1/2) | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20%PEG3350,200mM potassium citrate tribasic |
