7V34
The complex structure of soBcmB and its product 1d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03315 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 100.990, 100.990, 131.387 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.134 - 2.000 |
R-factor | 0.175625226054 |
Rwork | 0.174 |
R-free | 0.21014 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v3o |
RMSD bond length | 0.007 |
RMSD bond angle | 0.877 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.134 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.124 | 1.022 |
Number of reflections | 27428 | 1987 |
<I/σ(I)> | 23.3 | |
Completeness [%] | 100.0 | |
Redundancy | 26.8 | |
CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH 8.0, 1.6M Ammonium sulfate |