7V2U
The complex structure of SoBcmB and its product 2f
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03315 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 101.224, 101.224, 131.898 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.881 - 2.000 |
R-factor | 0.187814111561 |
Rwork | 0.186 |
R-free | 0.21494 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v2t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.881 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.124 | 1.234 |
Number of reflections | 27599 | 1988 |
<I/σ(I)> | 29 | |
Completeness [%] | 99.9 | |
Redundancy | 37.8 | |
CC(1/2) | 1.000 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES pH 7.0, 1 M Ammonium sulfate, 1 M potassium chloride |