7V1V
Difructose dianhydride I synthase/hydrolase (alphaFFase1) from Bifidobacterium dentium, ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.552, 156.970, 100.909 |
| Unit cell angles | 90.00, 109.93, 90.00 |
Refinement procedure
| Resolution | 49.690 - 1.960 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kq7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.690 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.098 | 0.432 |
| Rmeas | 0.116 | 0.518 |
| Rpim | 0.061 | 0.283 |
| Number of reflections | 206183 | 10181 |
| <I/σ(I)> | 10.3 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.2 |
| CC(1/2) | 0.994 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG3350, 0.2M di-sodium tartrate |
