7V1O
Crystal structure of mouse cytosolic sulfotransferase mSULT3A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 116.440, 116.440, 203.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.110 - 2.400 |
| R-factor | 0.2328 |
| Rwork | 0.231 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.643 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 29326 | 1732 |
| <I/σ(I)> | 22.3 | 2.1 |
| Completeness [%] | 89.9 | 60.2 |
| Redundancy | 9.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 3,350 200 mM LiSO4 20 mM DTT 100 mM Tris, pH 8.5 |
