7V1M
Structural basis for the co-chaperone relationship of sNASP and ASF1b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 |
Unit cell lengths | 71.951, 71.896, 93.427 |
Unit cell angles | 70.67, 70.64, 83.61 |
Refinement procedure
Resolution | 35.588 - 2.834 |
R-factor | 0.2097 |
Rwork | 0.202 |
R-free | 0.24030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v1l 5bnx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.950 |
High resolution limit [Å] | 2.834 | 6.130 | 2.850 |
Rmerge | 0.101 | 0.058 | 0.752 |
Rmeas | 0.118 | 0.068 | 0.890 |
Rpim | 0.060 | 0.035 | 0.471 |
Number of reflections | 37598 | 3786 | 3563 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 95.6 | 96.4 | 91.3 |
Redundancy | 3.6 | 3.7 | 3.3 |
CC(1/2) | 0.993 | 0.606 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8% v/v Tacsimate, pH 6.0, 20% w/v PEG 3350 |