7UYX

Structure of bacteriophage PA1c gp2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	60.955, 86.563, 71.879
Unit cell angles	90.00, 110.94, 90.00

Refinement procedure

Resolution	67.130 - 2.630
R-factor	0.2723
Rwork	0.271
R-free	0.30010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold 2 model
RMSD bond length	0.004
RMSD bond angle	0.537
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.130	2.740
High resolution limit [Å]	2.630	2.630
Rmerge	0.184	1.101
Rmeas	0.220	1.306
Rpim	0.119	0.694
Number of reflections	20612	2402
<I/σ(I)>	4.8	0.9
Completeness [%]	98.5	94
Redundancy	3.3	3.4
CC(1/2)	0.981	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris pH 8.5, 1.5 M Lithium sulfate