7UYH
Structure of the first OTU domain from Legionella pneumophila effector protein LotA bound to K6-linked diUb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 127.283, 127.283, 77.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.990 - 2.800 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7uyg 1ubq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.990 | 38.990 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.257 | 0.076 | 1.150 |
| Rmeas | 0.279 | 0.084 | 1.250 |
| Rpim | 0.108 | 0.033 | 0.483 |
| Total number of observations | 106091 | 3306 | 15505 |
| Number of reflections | 16316 | 596 | 2330 |
| <I/σ(I)> | 6.4 | 16.7 | 2 |
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 6.5 | 5.5 | 6.7 |
| CC(1/2) | 0.986 | 0.992 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350, 10% ethylene glycol, 0.2 M KSCN, 0.1 M MES pH 5.5 |
