7UYG
Structure of the first OTU domain from Legionella pneumophila effector protein LotA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.245, 86.245, 92.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.800 - 1.500 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.22050 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.769 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.070 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.035 | 0.819 |
| Number of reflections | 56168 | 2895 |
| <I/σ(I)> | 26.6 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG 2K MME, 0.2 M KI, 0.1 M MES pH 6.5 |
