7UYF
Human PRMT5:MEP50 structure with Fragment 4 and MTA Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-19 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.475, 137.782, 178.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.970 - 2.820 |
| Rwork | 0.269 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.970 | 3.070 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 29737 | 6659 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 98.5 | 99.5 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 281 | 10% PEG 4000, 100 mM Sodium Acetate, 100 mM Sodium Citrate pH 5.4 |
