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7UY1

HUMAN PRMT5:MEP50 COMPLEX WITH MTA and Fragment 5 Bound

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2018-10-17
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.00000
Spacegroup nameI 2 2 2
Unit cell lengths101.187, 138.210, 178.564
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution45.960 - 2.660
Rwork0.260
R-free0.30000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)NONE
RMSD bond length0.003
RMSD bond angle0.997
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0232)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.9602.910
High resolution limit [Å]2.6602.660
Rmerge0.0500.440
Number of reflections340548304
<I/σ(I)>25.3
Completeness [%]93.898.4
Redundancy55.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION291PEG/Buffer/Salt

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