7UY1
HUMAN PRMT5:MEP50 COMPLEX WITH MTA and Fragment 5 Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 101.187, 138.210, 178.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.960 - 2.660 |
Rwork | 0.260 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.997 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.960 | 2.910 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.050 | 0.440 |
Number of reflections | 34054 | 8304 |
<I/σ(I)> | 25.3 | |
Completeness [%] | 93.8 | 98.4 |
Redundancy | 5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | PEG/Buffer/Salt |