7UXX

Crystal structure of SARS-CoV-2 nucleocapsid protein C-terminal domain

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRUS BEAMLINE MANACA
Synchrotron site	LNLS SIRUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.3236
Spacegroup name	P 21 21 21
Unit cell lengths	43.615, 119.750, 128.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.810 - 1.850
Rwork	0.163
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c22
RMSD bond length	0.010
RMSD bond angle	1.598
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.810	1.916
High resolution limit [Å]	1.850	1.850
Number of reflections	58500	5760
<I/σ(I)>	9.33
Completeness [%]	100.0	99.98
Redundancy	2	2
CC(1/2)	0.997	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	291	100 mM Tris-HCl (pH 8.3), 30% PEG 4000, 0.2 M sodium acetate