7UXI

Structure of CDK2 in complex with FP19711, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	54.330, 65.800, 72.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.650 - 2.070
R-factor	0.2128
Rwork	0.210
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b38
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	2.140
High resolution limit [Å]	2.070	2.070
Rmerge	0.168	1.210
Number of reflections	16341	1600
<I/σ(I)>	9.4	2.2
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.996	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Tris pH 8.5, 25% w/v PEG 2,000MME