7UX9
Arabidopsis DDM1 bound to nucleosome (H2A.W, H2B, H3.3, H4, with 147 bp DNA)
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Number of Particle | 3788872 |
Software | Warp,cryoSPARC,EPU,Coot,PHENIX |
CTF correction | PHASE FLIPPING ONLY |